Plant tissue culture top ten books free download

Therefore, plant tissue culture has been introduced as a compulsory course in the Undergraduate and Postgraduate syllabi of all the Agricultural Universities, ICAR institutes and other plant science related educational organizations.

This book has been designed to benefit the students, the research scholars and the scientists for developing a level of self-confidence to conduct the experiments independently and can acquire the practical skills along with the basic know-how about the techniques being used.

Each chapter is devoted to a separate aspect of plant tissue culture and the chapters are arranged in the order of increasing technical complexity. The opening chapters present a brief historical survey of the field of plant tissue culture, a background in sterilization techniques.

The text deals with the experimental details of each and every technique. The protocols have been simplified legibly to include details and notes that we hope will help the user avoid unnecessary errors and confusion. All the applications of plant tissue culture have been very well discussed and the techniques associated with them described in detail.

This being a comp-lete book on Plant tissue culture will solve all types of problem of the users who will not have to use other resource books for the same purpose. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field.

Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.

What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science. Filled with illustrations of key concepts and references to up-to-date textbooks, papers, and reviews, this handy guidebook defines both the terms that come from plant tissue culture, and those that originate from plant anatomy, genetics, stress physiology, growth regular research, microbiology, and plant pathology.

Dictionary of Plant Tissue Culture is essential for anyone involved in tissue culture or using plant tissue culture systems for plant cloning, secondary metabolic production, plant pathology, and genetic manipulations. Plant Tissue Culture Author : B. Sathyanarayana Publisher : I.

This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. The book gives latest information on the current research work carried out in the field of plant tissue culture. The book is interdisciplinary in nature and will be of use to botanists, agriculturists, ecologists, biotechnologists and horticulturists.

Plant tissue culture PTC is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research.

PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection , clonal propagation Micropropagation , virus elimination Shoot Tip Culture , germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology Genetic Engineering or cell fusion Somatic Hybridization and Cybridization.

Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. White in , several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences.

More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments.

A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book.

This pioneering work is valuable for the students who are looking for fresh outlook and search. Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol. Plants cell tissue culture is a rapidly developing technology which holds promise of restructuring agricultural and forestry practices.

During the last two decades cell culture have made considerable advanced in the field of agriculture, horticulture, plant breeding, forestry, somatic cell genetics, phytopathology etc.

Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells that appear on cut surfaces when a plant is wounded and which gradually cover and seal the damaged area. Plant cells and tissue culture are often used for the production of primary and secondary metabolites.

Plant tissue cultures can be initiated from almost any part of a plant. The physiological state of the plant does have an influence on its response to attempts to initiate tissue culture.

The parent plant must be healthy and free from obvious signs of disease or decay. The source, termed explant, may be dictated by the reason for carrying out the tissue culture. Younger tissue contains a higher proportion of actively dividing cells and is more responsive to a callus initiation programme. The plants themselves must be actively growing, and not about to enter a period of dormancy.

Plant tissue culture is used widely in plant science; it also has a number of commercial applications. Tissue culture is employed in; micropropagation, elimination of pathogens from plant materials, germoplasm storage, production of somaclonal varients, embryo rescue, production of haploids, production of artificial seeds, production of secondary metabolities, production of transgenic plants etc.

Some of the fundamentals of the book are plant tissue culture, basic requirements for tissue culture laboratory, surface sterilization of explant materials, development of tissue culture techniques, principles of cell culture cell, special factors influencing growth and metabolism, media for culturing cells and tissues, sterilisation procedures, design and equipment of a tissue culture laboratory, isolation method for microorganisms for culture, culture preservation and stability, genetic modification of industrial microorganisms mutation etc.

The present book discuss about the methods, culture preservation and stability procedures, storage and transportation of plant cell tissue culture. This book is an invaluable resource for research workers, students, technocrats, entrepreneurs, institutional libraries etc. Do you want to know how to tissue culture plants and grow more in less space?

If so this how-to guide is for you. Plant tissue culture can be done at home without expensive lab grade gear. Fundamental principles: PTC depends upon 1. Totipotency :- It is the ability of plant cells to regenerate into a whole plant 2.

Plasticity :- It is the ability of plants to alter their metabolism, growth and development to best suit their environment 5. Explant Plant tissue cultures are generally initiated from multicellular tissue fragments, called explants, obtained from living plants.

Explants may originate from a wide range of plant tissues, such as… leaf, stem, root, petiole, hypocotyl, cotyledon, embryo, or meristem 6. By somatic embryogenesis 7. Transformation and gene expression.

In Advances in botanical research, volume Biotechnology of cereals ed. Shewry, P. Lazzeri and K. Edwards , pp. Academic Press, London. Donor-plant environment effects on regeneration from barley embryo-derived callus.

Crop Science, 39, —5. An improved media system for high regeneration rates from barley immature embryo-derived callus cultures of commercial cultivars. Crop Science, 42, —8. Experiments in plant tissue culture. Cambridge University Press, Cambridge. Total views ,